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EnglishVoice Age
Middle Aged (35-54)Accents
North American (General) North American (US General American - GenAM)Transcript
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next generation sequencing is a powerful tool that can provide comprehensive data for researchers focused on discovering genetic variants in select regions of the genome. To get useful data on specific variations in the genome, you need your screen to be comprehensive and capture the most information possible. However, sequencing and screening the whole genome is very costly and time consuming. Target in Richmond allows you to focus on specific areas of interest within the genome and provides the ability to sequence more samples at a lower cost. It also improves confidence in the data from better coverage death. Roche has enhanced and streamlined the target enrichment process with the development of the new hyper cap workflow. This workflow combines to best in class solutions Kappa Hyper prep and hyper plus library preparation and see Cap Easy Target in Richmond from a single trusted vendor for complete support throughout the project. This fast, flexible workflow eliminates the need for the expensive equipment, reduces cumbersome hands on processing and can be fully automated on many commercially available liquid handlers. And it is compatible with samples of varying input, amounts and quality because varying sample and put amounts can play a big role in the duplicate rate and percent of coverage for a researcher. We have optimized the hyper cap workflow toe work at very small DNA input amounts. Here you can see with different input amounts. Using the hyper Plus kid, we were able to maintain low duplicate rate and provide a high percentage of bases covered with at least 20 reads or more across a wide range of input amounts before using the hyper cap workflow. Custom design starts with nimble design software and easy to use interactive Web application that facilitates custom pro pool design. Nimble design software shortens turnaround time for custom designs and picks probes that comprehensively cover regions of interest through proven industry leading design algorithms. Alternatively, choose from Roche is growing C cap Shared Designs Library, a comprehensive menu of expert designed content that is available for multiple organisms and is compatible with streamlined hyper cap workflow. Once you and your sample are ready, we begin with nuclear acid isolation. To start the target enrichment process, Roche has NGS compatible protocols on the Magna pure family of automated nucleic acid extraction systems for any throughput. The use of this system further streamlines the ordering and support experience. The hyper cap workflow begins with D and a library preparation using Kappa Hyper prep or hyper plus library preparation kids. These library preparation kids have been designed and optimized to offer streamlined single tube library prep process. Benefits of this enhanced workflow include increased library yields and diversity, lo amplification bias, reduced duplication rates and improved sequencing uniformity over targeted regions of interest. In addition, this workflow employs the dual indexing strategy, enabling hire more reliable multiplexing in enrichment and sequencing. Following the library prep process, regions of interest are enriched by hybridizing sequencing libraries with C cap probe pools, leveraging millions of probes and overlapping tiling designs. See Capt. Pro Pools target and enrich up to 200 mega bases from any genome. This portion of the hyper cap workflow offers pre or post capture multiplexing, optimally configured and complete re agent kits to accommodate a variety of multiplexing needs with a customizable workflow for a variety of sample types and starting and put amounts. In addition, it has been further streamlined with the introduction of Rocha's new universal blocking all egos. These new only goats reduce workflow, complexity and hands on time by eliminating the need for adapter matched blocking only goes the introduction of Russia's new universal blocking only goes has further simplified the workflow. The's blocking only go saturates sequencing adapters, preventing cross hybridization with off target fragments and improve performance by increasing the percent of library fragments and regions of interest. And this streamlined workflow still out performs the alternative work flows. Here we see how are universal blocking only goes perform against only goes from supplier X. The Roche you BeOS demonstrated equal or better performance than Supplier X's blocking only goes, and uniformity was consistent across all blocking conditions tested. The enriched fragment pool is then amplified by PCR and enrichment. Success is measured by real time PCR, a control low side which is compatible with the light cycler family of high performance, real time PCR instruments. For any throughput level. Using the light cycler further streamlines the ordering and support experience. This enriched DNA pool is now ready for high throughput next gen sequencing on alumina based systems because hyper cap workflow has been streamlined libraries air ready to be sequenced in 1.5 days. The Roche hyper cap workflow simplifies target in Richmond with a fully integrated sample prep solution. It also ensures greater success with a wide range of sample and put in quality increases. Throughput using commercially available liquid handlers from several vendors and allows you to multi plex more samples with Kappa dual indexed adopters. And you'll save time by partnering with a single trusted vendor for ordering service and support. For more information on the hyper cap workflow, please visit sequencing dot Roche dot com.