British, England - Received Pronunciation (RP, BBC)
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for most scientific PCR cloning kit is an advanced positive selection system for high efficiency cloning of PCR products generated with any stable DNA preliminaries. Traditionally, any other DNA fragment either blunt or sticky ended could be successfully cloned using the kid, while blunt ended PCR products generated with proof reading enzyme can proceed immediately into the legation. Reaction with cloning Vector PCR products with sticky ends require a five minute blunting step for this purpose. Thie kit includes a proprietary blunting enzyme that removes three prime overhangs and fills in five prime overhangs using the nuclear tides included in the reaction.