Technical summary (isothermal PCR)
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molecular biology techniques such as polymerase chain reaction or PCR, Real time PCR and DNA hybridization have been developed and become popular for pathogen identification. Although these technologies can detect low number of bacterial cells, they usually need several hours. Moreover, such techniques required prior cultural enrichment and bacterial DNA isolation, preparation of enzyme reaction mixtures and expensive equipment. Therefore, most of these techniques are not suitable for on site detection. Nevertheless, loot mediated ice a thermal amplification were. LAMP is a simple, cost effective and rapid method that is often used as an alternative to PCR based methodologies and pathogen detection.