Basics of Chromatography - Technologic - Technology - Education - Scientific - Science - eLearning - Training
English (North American)
Middle Aged (35-54)
Note: Transcripts are generated using speech recognition software and may contain errors.
What is chromatography? Chromatography is an analytical technique of separating complex mixtures consisting of multiple components and how does chromatography work. Let's take a look at a simple experiment paper. Chromatography Step one, prepare the paper and mark a dot on it in pen ink. Step two. Place the paper into a jar filled with some solvent water this time and soak the edge of the paper. Step three. When the water spreads up through the paper, the black ink is separated into each die. Through this experiment we can define the essential terms used in chromatography. What our mobile phase and stationary phase mobile phase is used to carry the mixture along with the stationary phase, stationary phase remains static in one place where separation occurs, the stationary phase varies depending on the types of chromatography used. What's the difference among chromatography, chromatograph and chroma to Graham. Chromatography is a technology for the separation of a mixture into its individual components between the mobile and stationary phases. Chromatograph is an analytical instrument analyzing organic compounds based on chromatography. Chroma to graham is visual data of chromatograph shown in a graph of detectors, signal output versus illusion time that can be interpreted for the channel. It's what are the types of chromatography. Chromatography can be categorized into gas chromatography and liquid chromatography, depending on the phase type of mobile phase used. Gas chromatography, gas is used as mobile phase compounds under 500 molecular weight can be analyzed by G. C. And G. C. Is suitable for analysis of thermally stable volatile compounds. On the other hand, L. C. Uses liquid as mobile phase and there are no limitations of molecular weight that L. C. Can analyze. L. C. Is suitable for analysis of non volatile and soluble compounds. G. C. Can be classified into two types again, G L. C and G S. C G L. C. Gas liquid Chromatography uses liquid films coated on the support or inner wall as the stationary phase. Two separate compounds by partition chromatography, G. S. C, which is gas solid chromatography uses solid porous particles as the stationary phase two separate compounds by absorption chromatography. Yeah, move to the liquid chromatography. L. C. Can be categorized as L. L. C. And L. S. C. L. L. C. Which stands for liquid liquid chromatography uses partition chromatography method. L. S. C. Can be classified into three types absorption ion exchange and size exclusion chromatography. Let's move to partition chromatography partition chromatography is one of the separation techniques using the difference of partition coefficient between two liquid phases, salut components dissolved in liquid phase which is coated on solid support surface partition coefficient depends on the soluble itty difference. So the more soluble it is the later it comes out absorption chromatography. It is a separation technique based on the absorption and description interaction between stationary phase and components ion exchange chromatography. It is a separation technique based on the interaction between the charged compound and an opposite lee charged stationary phase. There are two types of ion chromatography and ion exchange and cat ion exchange. Mhm size exclusion chromatography. It separates compounds on the basis of different components sizes. Sec is also called GPC gel permeating chromatography. Larger molecules are eluded from the column earlier and smaller molecules later on. So this is the brief classification of chromatography types, and you can see how chromatography is classified at a glance. Chromatography is widely used in industrial processes. To purify chemicals, test trace quantities of substances separate Cairo compounds and quality control test products. Mhm. Want to learn more about chromatography, please stay tuned on young and crow mass channel. Mhm mm.
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